Description
ANXA7, also known as annexin A7, is a member of the annexin family of calcium-dependent phospholipid binding proteins. The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. Structural analysis of the protein suggests that Annexin A7 is a membrane binding protein with diverse properties including voltage sensitive calcium channel activity, ion selectivity and membrane fusion. Recombinant human ANXA7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography